TABLE 1.
Oligonucleotide primers for PCR amplification of adenovirus species
| Species | Primer | Polarity | Gene | Gene region | Position (nt no.)a | Sequence (5′→3′) | Amplicon size (bp)b |
|---|---|---|---|---|---|---|---|
| A to F | Ad1 | + | Hexon | 1834–1853 | TTCCCCATGGCICAYAACAC | 482 | |
| Ad2 | − | 2315–2296 | CCCTGGTAKCCRATRTTGTA | ||||
| A | AdA1 | + | Fiber | Tail | 29392–29414 | GCTGAAGAAMCWGAAGAAAATGA | 1444–1537 |
| AdA2 | − | Knob | 30928–30908 | CRTTTGGTCTAGGGTAAGCAC | |||
| B | AdB1 | + | Fiber | Tail | 4573–4595 | TSTACCCYTATGAAGATGAAAGC | 670–772 |
| AdB2 | − | Knob | 5242–5220 | GGATAAGCTGTAGTRCTKGGCAT | |||
| C | AdC1 | + | Fiber | Flank | 333–354 | TATTCAGCATCACCTCCTTTCC | 1988–2000 |
| AdC2 | − | Flank | 2320–2301 | AAGCTATGTGGTGGTGGGGC | |||
| D | AdD1 | + | Fiber | Flank | 5–25 | GATGTCAAATTCCTGGTCCAC | 1205–1221 |
| AdD2 | − | Flank | 1219–1198 | TACCCGTGCTGGTGTAAAAATC | |||
| E | AdE1 | + | Fiber | Tail | 48–67 | TCCCTACGATGCAGACAACG | 967 |
| AdE2 | − | Knob | 1014–994 | AGTGCCATCTATGCTATCTCC | |||
| F | AdF1 | + | Fiber | Shaft | 1734–1754 | ACTTAATGCTGACACGGGCAC | 541–586 |
| AdF2 | − | Shaft | 2274–2253 | TAATGTTTGTGTTACTCCGCTC |
a
Nucleotide (nt) numbering for Ad group-specific primers based on published hexon gene sequence of Ad3 (41); nucleotide numbering for Ad species-specific primers based on published fiber gene sequences of Ad12 (49), Ad7 (26), Ad5 (7), Ad8 (39), Ad4 (17), and Ad40 (27).
b
Predicted amplicon size range based on previously published nucleotide sequences of representative Ad serotypes for each species.