TABLE 3.

Confirmation of results for Ad isolates with discrepant results by species-specific multiplex PCR assays

Isolate	Date collected (mo/day/yr)	Location	Original identificationa		Multiplex PCR assaysb		Repeat identificationc
			Species	Serotype	Fiber	Hexon	Species	Serotype
V-1707	5/3/84	Alabama	A	Ad18	C	C	C	Ad2
V-2362	8/16/91	New Mexico	A	Ad31	D	D	D	Ad36
99018072	NDd	Brazil	B	Ad11	B	Neg	B	Ad11
V-2181	12/20/88	New York	B	Ad11	B	Neg	B	Ad11
98034069	X/X/95	Egypt	B	Ad14	B	Neg	B	Ad14
V-2167A	9/8/85	Tennessee	B	Ad21/H21 + 35	B	Neg	B	Ad21 + 35
RU-8176	2/24/77	Tennessee	B	Ad21/H21 + 35	B	Neg	B	Ad21 + 35
V-2079A	4/30/87	Canada	B	Ad35	B	Neg	B	Ad35
V-2064A	1/30/87	Colorado	B	Ad7	B	Neg	B	Ad7
V-2215	7/26/89	Pennsylvania	C	Ad1	D	D	D	Ad19
V-375-4	8/28/89	El Salvador	D	Ad39	A	A	A	Ad31
V-2158B	6/22/88	Maryland	F	Ad40	A	A	A	Ad31
V-1533-18	11/24/81	Arizona	F	Ad40	A	A	A	Ad31
V-2159A	6/29/88	Maryland	F	Ad40	D	D	D	Ad9

^aOriginal identification of Ad isolates made by hemagglutination inhibition (23) and/or neutralization (21) assay. 

^bAd species-specific hexon multiplex PCR procedure performed as described by Pring-Åkerblom et al. (42). 

^cRepeat identification of Ad isolates made by microneutralization assay with type-specific horse antisera. 

^dND, not determined.