Figure 1.

Characterization of Duplex-Repair using capillary electrophoresis. (A) overview of Duplex-Repair vs. conventional ER/AT methods. (B) Schematic of the major products of various synthetic duplexes subjected to each step of Duplex-Repair and conventional ER/AT as determined by capillary electrophoresis (raw traces are in Fig S2). The non-fluorophore-tagged ends of the synthetic molecules are depicted, and fragment sizes are drawn to scale. Duplexes demarcated by asterisks (*) do not contain fluorophores and were not directly observed by capillary electrophoresis; however, their presence is predicted due to the characterized activities of UDG and FPG. Regions of strand resynthesis are illustrated in light blue.