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. 2022 Jan 4;38(1):110187. doi: 10.1016/j.celrep.2021.110187

Figure 1.

Figure 1

Characterization of the C. albicans-containing invasion pocket

(A–C) Confocal micrographs of short TR146 invasion pockets induced by wild-type C. albicans. In these and subsequent images the entire C. albicans was stained with calcofluor white (CFW) and those portions of the fungus outside the invasion pocket with concanavalin A (ConA). The PM was identified by expression of PM-RFP. Here and in all subsequent images, actin refers specifically to F-actin, which was stained with phalloidin (A); LAMP-1 immunostaining (B); PtdIns(3)P detected by expression of PX-GFP (C).

(D–F) Micrographs of RAW264.7 dectin-1 macrophages with frustrated phagosomes containing C. albicans. Micrographs show F-actin (D); LAMP-1 (E); PtdIns(3)P (F).

(G–I) Micrographs of long TR146 invasion pockets containing wild-type C. albicans. Micrographs show F-actin (G); LAMP-1 (H); PtdIns(3)P (I). Images are representative of ≥3 experiments of each type. All scale bars represent 5 μm.