Figure 3.

Candidalysin causes [Ca²⁺]_c oscillations

(A–C) TR146 cells were transfected with GCaMP6s-GFP and incubated with 30 μM candidalysin in the presence (A, top row, and B) or absence of extracellular Ca²⁺ (A, bottom row, and C). GCaMP6s fluorescence was monitored every 20 s for 12 min, followed by addition of thapsigargin (TG, 500 nM) for an additional 3 min. [Ca²⁺]_c changes, monitored using GCaMP6s, in 12 representative cells in response to candidalysin treatment in the presence of extracellular Ca²⁺, followed by TG. (C) [Ca²⁺]_c changes of 12 representative cells after candidalysin treatment in the absence of extracellular Ca²⁺, followed by addition of TG. Images and graphs are representative of at least 3 experiments of each type. Scale bars represent 20 μm.