TABLE 1.
Summarized results of PCR and DNA dot blot analyses of strains belonging to Vibrio spp. using primers or probes for ompW and toxR genes
| Analysis no(s). | Strain | Sourcea | Detectionb of:
|
|||
|---|---|---|---|---|---|---|
|
ompW by:
|
toxR by:
|
|||||
| PCR | Dot blot | PCR | Dot blot | |||
| 1 | V. cholerae O1 | 14035 (ATCC) | + | + | + | + |
| 2 | V. cholerae O1 | 39315 (ATCC) | + | + | + | + |
| 3–8 | V. cholerae O1 | Lab stock | + | + | + | + |
| 9–11 | V. cholerae O139 | Lab stock | + | + | + | + |
| 12–13 | V. cholerae (rough) | Lab stock | + | + | + | + |
| 14 | V. cholerae non-O1/non-O139 | 25872 (ATCC) | + | + | + | + |
| 15 | V. cholerae non-O1/non-O139 | 25874 (ATCC) | + | + | + | + |
| 16–18 | V. cholerae non-O1/non-O139 | Lab stock | + | + | + (2/3)c | + |
| 19 | V. mimicus | 33653 (ATCC) | − | +W | − | + |
| 20 | V. mimicus E13072 | NICED | − | − | − | − |
| 21 | V. mimicus 4208 | NICED | − | − | − | + |
| 22 | V. mimicus NT 2310 | NICED | − | − | − | − |
| 23 | V. mimicus 1 | NICED | − | − | − | − |
| 24 | V. mimicus 4053 | NICED | − | − | − | − |
| 25 | V. vulnificus | 33816 (ATCC) | − | − | − | − |
| 26 | V. vulnificus | 27562 (ATCC) | − | − | − | − |
| 27–28 | V. vulnificus | NICED | − | − | − | − |
| 29 | V. alginolyticus | 17749 (ATCC) | − | − | − | + |
| 30 | V. aestuarianus | 35048 (ATCC) | − | − | − | + |
| 31 | V. fluvialis | 33809 (ATCC) | − | − | − | + |
| 32 | V. furnissii | 35016 (ATCC) | − | − | − | +W |
| 33 | V. furnissii | NICED | − | − | − | − |
| 34 | V. proteolyticus | 15338 (ATCC) | − | − | − | +W |
| 35 | V. anguillarum | 19264 (ATCC) | − | − | − | − |
| 36 | V. anguillarum 943 | 43305 (ATCC) | − | − | − | − |
| 37 | V. anguillarum 944 | 43306 (ATCC) | − | − | − | − |
| 38 | V. anguillarum | NICED | − | − | − | − |
| 39 | V. carchariae | 35084 (ATCC) | − | − | − | − |
| 40 | V. tubiashii | 19109 (ATCC) | − | − | − | − |
| 41 | V. natriegens | 14048 (ATCC) | − | − | − | − |
| 42 | V. salmonicida | 43839 (ATCC) | − | − | − | +W |
| 43 | V. splendidus | 33125 (ATCC) | − | − | − | + |
| 44 | V. nereis | 25917 (ATCC) | − | − | − | − |
| 45 | V. parahaemolyticus | 2210001 (RIMD) | − | − | − | − |
| 46–51 | V. parahaemolyticus | NICED | − (6/6) | − (6/6) | − (6/6) | − (5/6)d |
| 52 | V. tyrogens | RS | − | − | − | − |
| 53 | V. tyrogens | RS | − | − | − | − |
| 54 | V. metschnikovii | RS | − | − | − | − |
| 55 | V. metschnikovii | NICED | − | − | − | − |
| 56 | V. metschnikovii | STM | − | − | − | − |
| 57 | V. hollisae | NICED | − | − | − | − |
| 58 | V. proteus | RS | − | − | − | − |
a
Lab stock, strains available as stock culture at the Bose Institute, Calcutta, India; NICED, National Institute of Cholera and Enteric Diseases, Calcutta, India; RIMD, Research Institute of Microbial Diseases, Osaka, Japan; RS, R. Sakazaki, Tokyo, Japan; STM, School of Tropical Medicine, Calcutta, India.
b
+, Detectable; +W, weakly detectable; −, not detectable.
c
One strain gave a negative result.
d
One strain gave a weakly positive result.