Fig. 7. R273C mut-p53 confers more aggressive phenotypes in vitro and in vivo.

A Colony formation assays were plated using LNCaP- or C4-2-derived cell lines (R273C or R273H) and then harvested to assess colony formation. Graphs represent at least four independent experiments in technical triplicate. Students t-test. B LNCaP- or C4-2-derived cell lines (R273C or R273H) were plated for clonogenic survival and then treated with the indicated dose of irradiation after 24 h. Graphs represent at least three independent experiments in triplicate. 2-way ANOVA. C LN-pLPLUC, LN-R273C, or LN-R273H cells were subcutaneously injected into immunocompromised (Nu/Nu) male mice and the time until tumor take reached 100 mm³ or castration surgery was performed between 100–150 mm³ (Mantel–Cox Log-Rank; LN-R273C N = 18; LN-R273H N = 11). D The doubling time from tumor take to tumor endpoint was measured in untreated and castrated male mice (one-way ANOVA with Tukey’s multiple comparisons test). E Schematic of study findings.