Extended Data Fig. 7. HELQ weakly displaces RAD51 from ssDNA.

a, Representative EMSA gel showing RAD51 binding to ssDNA in the absence and presence of HELQ. Reactions were incubated at 37 °C for 20 min. b, Quantification of experiments such as shown in a. n = 6 independent experiments; mean ± S.E.M. c, Representative EMSA gel showing RAD51 binding to dsDNA in the absence and presence of HELQ. Reactions were incubated at 37 °C for 20 min. d, Quantification of experiments such as shown in c. n = 3 independent experiments; mean ± S.E.M. e, Schematic of the experimental set up of single-molecule FRET-based RAD51 removal assay. f, Representative kymographs of removal of Alx–RAD51 pre-bound to gapped DNA in the presence and the absence of 50 nM HELQ or HELQ(K365M). g, Removal of Alx–RAD51 measured from gapped ssDNA as a function of time in indicated conditions. Traces represent individual DNA molecules (n ≥ 3).