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. 2021 Dec 22;601(7892):268–273. doi: 10.1038/s41586-021-04261-0

Extended Data Fig. 3. RPA inhibits HELQ DNA unwinding activity.

Extended Data Fig. 3

a, SDS-PAGE gel (4-12%) showing purified recombinant human RPA-mRFP1 from E. coli. The gel was stained with CBB. Single preparation of RPA-mRFP1 was used in this study. b, Representative gel of DNA unwinding assay of D-loop with HELQ in the absence and presence of RPA (20 nM). c, Quantification of experiments such as shown in b. n = 3 independent experiments; mean ± S.E.M. d, Representative gel of DNA unwinding assay of 3′-overhang with HELQ (1 nM) and indicated concentrations of RPA. e, Quantification of experiments such as shown in d. n = 3 independent experiments; mean ± S.E.M. f, Representative gel of DNA unwinding assay of 3′-overhang with HELQ (3 nM) and indicated concentrations of RAD51, in the absence and presence of RPA (20 nM). g, Quantification of experiments such as shown in f. n = 6 independent experiments; mean ± S.E.M. h, Representative gel of DNA unwinding assay of D-loop with HELQ (3 nM) and indicated concentrations of RAD51, in absence and presence of RPA (20 nM). i, Quantification of experiments such as shown in h. n = 5 independent experiments; mean ± S.E.M.