Skip to main content
. 2021 Nov 10;41(3):400–413. doi: 10.1038/s41388-021-02095-8

Fig. 8. ALKBH5-TRAF1 regulated NF-κB and MAPK pathways in MM.

Fig. 8

A Western blots of TRAF1, NF-κB p105, P-NF-κB p105 (Ser932), NF-κB p50, NF-κB p65, BRAF, p-BRAF (Ser445), p-MEK1/2 (Ser221), p-ERK1/2 (Thr202/Tyr204), c-Myc, and p-c-Fos (Ser32) in RPMI-8226 cells with or without TRAF1 KD. Cyclophilin B was used as a loading control. B GSEA plots show enriched gene sets of NF-κB and MAPK signaling pathways and MAPK downstream targets in ALKBH5-High (top 50%) vs. ALKBH5-Low (bottom 50%) groups in a cohort of patients with MM (GSE26760 [34]). C, D Western blots of NF-κB and MAPK pathway molecules in MM cells upon ALKBH5 KD (C) and TRAF1 restoration (D). Cyclophilin B was used as a loading control. E Proposed model showing the role of ALKBH5 in MM pathogenesis.