Fig. 4.

A) Rho-incorporated Lipo-S1 vs C6-NP in 1:1 ratio in the ELISA ACE2 experiments. Fluorescent red signal (Ex 544 nm, Em 668 nm) was read for Rho and indicates the binding of Lipo-S1. Conversely, the competition/inhibition by C6-NP (C6-TNP-1-4 and C6-NP-0) was detected from the green fluorescence (Ex 488 nm, Em 510 nm). (∗) and (∗∗) significantly (∗∗p < 0.01 and ∗p < 0.05 vs Lipo-S1, n = 4) by one-way ANOVA and post-hoc Dunnett's test. Statistical analysis was also performed among the candidates, C6TNP-1 showed statistical significance against C6-NP-0 (∗∗∗p < 0.001 vs C6-NP-0 one-way ANOVA and post-hoc Dunnett's test).