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. 2022 Jan 13;231:114121. doi: 10.1016/j.ejmech.2022.114121

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Fig. 9 — Uptake assays for NP. Panels show live cell confocal fluorescence microscopy images of Vero E6 cells after exposure to targeted (TNP-1) and nontargeted (NP-0) Rho-loaded NP. Panels A and E: Rho-TNP-1 and Rho-NP-0, respectively. Panels B and F: visualization of ACE2 cell receptors (in green) overexposed by lenti-hACE2. Panels C and G: merge images of Vero E6 cells incubated for 3 h with Rho-TNP-1 and Rho-NP-0, respectively. Panels D and H: blue fluorescence of the nuclei stained with DAPI dye. Cellular binding/uptake visualized by overlaying images (scale bar 20 nm).