Figure 6.

SAMHD1 promoted NAb responses at 28 dpi in a sex-dependent manner. Plasma samples were collected in FV-infected mice pre-treated with LPS. (A) FV-specific IgG binding titers were determined by ELISA using plates coated with native F-MLV virions. Binding titers were determined based on an arbitrary absorbance cut-off (0.25) from a best-fit curve and plotted in (B). (C) The data were partitioned between male and female mice. (D) Neutralizing antibody responses were evaluated by serially diluting plasma 4-fold with a known amount of F-MLV and plating on Mus dunni cells for 2 days. Foci of infection were counted using ImageJ software and neutralization curves were constructed. Shown here were the average %infection values for WT versus SAMHD1 KO mice. (E) 80% inhibitory concentrations (IC80) were calculated and compared between the cohorts of mice. (F) IC80 were subdivided between male and female mice. For all panels, each data point corresponds to a mouse. The data were pooled from 2 independent cohorts of WT and SAMHD1 KO mice. Experiment 1 had 7 WT and 6 SAMHD1 KO mice, and experiment 2 had 7 WT and 5 SAMHD1 KO mice. Log-transformed data were analyzed using a 2-tailed unpaired Student’s t-test. *, p<0.05; **, p<0.01; NS, not significant (p>0.05).