FIGURE 5.

Loss of ABCB10 resulted in altered metabolic activity in Jurkat cells. (A) Control C1 and ABCB10 null A3 cells were incubated +/− anti-CD3/CD28 for 72 hr and then plated onto Seahorse 96 well plates precoated with CellTek (Corning). Cells were centrifuge onto plates at 1500 × g for 20 minutes and oxygen consumption (OCR) determined by Seahorse as described in Materials and Methods. (B) External acidification (ECAR) was determined on cells as in A. (C) Basal respiration, ATP-linked respiration, Proton leak, Maximal respiratory capacity and Respiratory reserve were determined as described (27). A representative analysis is shown. Error bars represent SEM of 12 biologic replicates.