KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Anti-mouse CD8a BUV395 (53-6.7) | BD Biosciences | Cat# 563786, RRID: AB_2732919 |
| Anti-mouse CD8a PerCP-Cy5.5 (53-6.7) | Tonbo Biosciences | Cat# 65-0081, RRID:AB_2621882 |
| Anti-mouse CD8a PE (53-6.7) | Tonbo Biosciences | Cat# 50-0081, RRID:AB_2621741 |
| Anti-Mouse CD45.1 violetFluor 450 (A20) | Tonbo Biosciences | Cat# 75-0453, RRID:AB_2621949 |
| Anti-Mouse CD45.1 PE-Cyanine7 (A20) | Tonbo Biosciences | Cat# 60-0453, RRID:AB_2621850 |
| Anti-Mouse CD45.2 violetFluor 450 (104) | Tonbo Biosciences | Cat# 75-0454, RRID:AB_2621950 |
| Anti-Mouse CD45.2 PE-Cyanine7 (104) | Tonbo Biosciences | Cat# 60-0454, RRID:AB_2621851 |
| Anti-Mouse CD45.2 FITC (104) | Tonbo Biosciences | Cat# 35-0454, RRID:AB_2621692 |
| Anti-human/mouse/rat CD278 (ICOS) PE (C398.4A) | BioLegend | Cat# 313508, RRID:AB_416332 |
| Anti-human/mouse/rat CD278 (ICOS) APC (C398.4A) | BioLegend | Cat# 313510, RRID:AB_416334 |
| Anti-mouse CD44 BV786 (IM7) | BD Biosciences | Cat# 563736, RRID:AB_2738395 |
| Anti-mouse CD44 BV605 (IM7) | BD Biosciences | Cat# 563058, RRID:AB_2737979 |
| Anti-mouse CD62L BV786 (MEL-14) | BD Biosciences | Cat# 564109, RRID:AB_2738598 |
| Anti-mouse CD62L BV605 (MEL-14) | BioLegend | Cat# 104438, RRID:AB_2563058 |
| Anti-mouse KLRG1 PE-Cyanine7 (2F1) | Thermo Fisher Scientific | Cat# 25-5893-82, RRID:AB_1518768 |
| Anti-mouse KLRG1 BV711 (2F1) | BD Biosciences | Cat# 564014, RRID:AB_2738542 |
| Anti-Mouse CD127 BUV737 (SB/199) | BD Biosciences | Cat# 612841, RRID:AB_2870163 |
| Anti-Mouse CD69 PE (H1.2F3) | Thermo Fisher Scientific | Cat# 12-0691-83, RRID:AB_465733 |
| Anti-Mouse CD69 PE-CF594 (H1.2F3) | BD Biosciences | Cat# 562455, RRID:AB_11154217 |
| Anti-mouse CD103 BV510 (M290) | BD Biosciences | Cat# 563087, RRID:AB_2721775 |
| Anti-mouse CD103 APC (2E7) | BioLegend | Cat# 121414, RRID:AB_1227502 |
| Anti-mouse CD275 (B7-RP1, ICOSL, B7H2) PE (HK5.3) | BioLegend | Cat# 107405, RRID:AB_2248797 |
| Anti-mouse LPAM-1 (Integrin a4b7) PE (DATK32) | BioLegend | Cat# 120606, RRID:AB_493267 |
| Anti-Mouse CD45 APC (30-F11) | Tonbo Biosciences | Cat# 20-0451, RRID:AB_2621573 |
| Anti-Mouse CD19 PE-Cyanine7 (1D3) | Tonbo Biosciences | Cat# 60-0193, RRID:AB_2621840 |
| Anti-Mouse CD45R/B220 BUV395 (RA3-6B2) | BD Biosciences | Cat# 563793, RRID:AB_2738427 |
| Anti-Mouse Va2 TCR APC (B20.1) | BD Biosciences | Cat# 560622, RRID:AB_1727582 |
| Ghost Dye Red 780 Viability Dye | Tonbo Biosciences | Cat # 13-0865-T100 |
| InVivoMAb anti-mouse CD3ε (145-2C11) | BioXCell | Cat# BE0001-1, RRID:AB_1107634 |
| InVivoMAb anti-mouse CD28 (37.51) | BioXCell | Cat# BE0015-1, RRID:AB_1107624 |
| InVivoMAb anti-mouse ICOSL (CD275) (HK5.3) | BioXCell | Cat# BE0028, RRID:AB_1107566 |
| InVivoMAb rat IgG2a isotype control (2A3) | BioXCell | Cat# BE0089, RRID:AB_1107769 |
| TotalSeq™-A0157 anti-mouse CD45.2 (104) | BioLegend | Cat# 109853, RRID:AB_2783051 |
| TotalSeq™-A0171 anti-human/mouse/rat CD278 (ICOS) (C398.4A) | BioLegend | Cat# 313555, RRID:AB_2800824 |
| TotalSeq™-A0301 anti-mouse Hashtag 1 (M1/42; 30-F11) | BioLegend | Cat# 155801, RRID:AB_2750032 |
| TotalSeq™-A0302 anti-mouse Hashtag 2 (M1/42; 30-F11) | BioLegend | Cat# 155803, RRID:AB_2750033 |
| TotalSeq™-A0303 anti-mouse Hashtag 3 (M1/42; 30-F11) | BioLegend | Cat# 155805, RRID:AB_2750034 |
| TotalSeq™-A0309 anti-mouse Hashtag 9 (M1/42; 30-F11) | BioLegend | Cat# 155817, RRID:AB_2750042 |
| TotalSeq™-A0310 anti-mouse Hashtag 10 (M1/42; 30-F11) | BioLegend | Cat# 155819, RRID:AB_2750043 |
| TotalSeq™-A0311 anti-mouse Hashtag 11 (M1/42; 30-F11) | BioLegend | Cat# 155821, RRID:AB_2750136 |
| Bacterial and virus strains | ||
| Lymphocytic choriomeningitis virus (LCMV) Armstrong strain | Dr. R. Ahmed, Emory University | N/A |
| Listeria monocytogenes-GP33 (LM-gp33) Generated by Dr. Hao Shen (UPenn) first reported PMID: 11323695 | Dr. R. Ahmed, Emory University | N/A |
| Influenza A-PR8-gp33. Generated by Dr. Ryan Langois (UMN), first reported in PMID: 31235953. | Dr. D. Masopust, (UMN) | N/A |
| Chemicals, peptides, and recombinant proteins | ||
| Collagenase, Type 1 | Worthington | Cat# LS004197 |
| Percoll | GE Healthcare | Cat# 17-0891-09 |
| Dithioerythritol | EMD Millipore | Cat# 233152-5GM |
| RPMI 1640 | Corning | Cat# 10-040-CV |
| HBSS 10x | Corning | Cat# 20-021-CV |
| Fetal Bovine Serum | Atlas Biologicals | Cat# FS-0500-AD |
| L-Glutamine, 100x, Liquid | Corning | Cat# 25-005-CI |
| Penicillin/Streptomycin | Gibco | Cat# 15070063 |
| DMEM | Gibco | Cat# 11995065 |
| Critical commercial assays | ||
| CD8a+ T cell Isolation Kit, Mouse | Miltenyi Biotec | Cat# 130-104-075 |
| BD Cytofix/Cytoperm Solution Kit | BD Biosciences | Cat#554714 |
| Deposited data | ||
| scRNA-seq of co-transferred WT and Icos−/− P14 T cells | this paper | GEO: GSE185342 |
| TRM single-cell time course study | Kurd et al., 2020 | GEO: GSE131847 |
| mouse cell atlas study | Han et al., 2018 | GEO: GSE108097 |
| Experimental models: Cell lines | ||
| Plat-E | Cell Biolabs | Cat#RV-101, RRID: CVCL_B488 |
| Experimental models: Organisms/strains | ||
| Mouse: B6.SJL-PtprcaPepcb/BoyCrCrl (B6-CD45.1) | NCI Charles River | Cat# CRL:564, RRID:IMSR_CRL:564 |
| Mouse: C57BL/6NCrl (B6-CD45.2) | NCI Charles River | Cat# CRL:027, RRID:IMSR_CRL:027 |
| Mouse: P14 | Dr. R. Ahmed, Emory University | N/A |
| Mouse: B6.129P2-Icostm1Mak/J (ICOS-KO) | The Jackson Laboratory | Cat# JAX:004859, RRID:IMSR_JAX:004859 |
| Mouse: B6.129P2-Icosltm1Mak/J (ICOSL-KO) | The Jackson Laboratory | Cat# JAX:004657, RRID:IMSR_JAX:004657 |
| Mouse: B6(C)-Klf2tm1.1Khog/JmsnJ (KLF2-GFP) | Generated in lab (Weinreich et al., 2009) | RRID:IMSR_JAX:036331 |
| Mouse: ICOS-YF | Dr. D. Campbell (Mittelsteadt et al., 2021) | N/A |
| Oligonucleotides | ||
| N/A | ||
| Recombinant DNA | ||
| MIGR1 (pMIGR-EV) | Dr. W. Pear (Addgene) | Cat# 27490 |
| pMIGR-mICOS | in-house | N/A |
| Software and algorithms | ||
| GraphPad Prism v9.2.0 | GraphPad Software | RRID:SCR_002798 |
| FlowJo v10.8 | BD Biosciences | RRID:SCR_008520 |
| BD FACSDiva | BD Biosciences | RRID:SCR_001456 |
| Cell Ranger v6.0.1 | 10xGenomics | RRID:SCR_017344 |
| Seurat v 3.0.3.9039 | https://satijalab.org/seurat/get_started.html | RRID:SCR_016341 |
| ggplot2 | https://cran.r-project.org/web/packages/ggplot2/index.html | RRID:SCR_014601 |
| MAGIC | van Dijk et al., 2018 | N/A |
| Other | ||