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. 2021 Sep 26;72(1):136–148. doi: 10.1007/s12031-021-01916-5

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — Co-transplantation of miR-615 agomir with gel enhanced neovascularization and reduced the astrocyte activation after brachial plexus avulsion. a Immunofluorescence photos of angiogenesis around avulsed site in the ipsilateral C6 segment. b Analysis of neurovascular areas at 6 weeks after surgery. The PBS rats exhibited few angiogeneses, while the rats in miR-615 agomir and miR-615 agomir+gel groups displayed a significant increase of neovascularization. c Immunofluorescence photos of GFAP around avulsed site in the ipsilateral C6 segment. d Relative area fraction of GFAP at 6 weeks after surgery. The miR-615 agomir and miR-615 agomir+gel groups rats exhibited few astrocyte activations, while the rats in PBS, NC-agomir, and gel groups displayed a significant increase of astrocyte activation. Data are presented as the mean ± SD (one-way analysis of variance followed by the least significant difference post hoc test). *p < 0.05, **p < 0.01, ***p < 0.001, vs. PBS group. Scale bar (a and c) = 200 μm