Fig. 7. CircTIAM1 sponges miR-646 to promote tumorigenesis in vivo.
A 1 × 107 TPC-1 cells were injected into nude mice, and representative images of subcutaneous tumors were photographed after five weeks. B Tumor weights of mice were measured in each group after the mice were dissected (n = 6 per group). Data represent the mean ± SEM. *p < 0.05. C Tumor volumes were recorded every week after injection. Data represent the mean ± SEM (n = 6). *p < 0.05. D Immunohistochemistry assay was conducted to exhibit HNRNPA1 protein levels in tumors, and H&E staining was performed to show the tumor structure. Scale bars = 100 μm. E HNRNPA1 expression of tumors from different groups was detected at the protein level by Western blot assay. F HNRNPA1 expression of tumors was detected at the RNA level by qRT-PCR assay. G Schematic representation of the hypothesis of the circTIAM1/miR-646/HNRNPA1 axis.