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. 2022 Jan 12;7:3. doi: 10.1038/s41536-021-00196-2

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — Migration plots of trajectories in response to A medium and B, C rmShh gradient using macrophages cultured from the bone marrow of control and SmoKO mice. D Macrophage migration is represented as forward migration index (FMI). Representative immunofluorescence stains of actin (green) and nuclei (red) of bone marrow-derived macrophages cultured from E control and F SmoKO mice treated with vehicle, CCL2, or rmShh. Arrows indicate filopodia. G Representative immunofluorescence stain of CCR2 (red) and nuclei (Hoechst, blue) of bone marrow-derived macrophages cultured from control or SmoKO mice and treated with PBS, rmShh, or MCP. H Quantitative RT-PCR of CCR2 expression using RNA collected from control or SmoKO bone marrow-derived mouse macrophages and treated with PBS, Shh, MCP1, or CXC3L1. *P < 0.05 compared to PBS vehicle control, n = 6 individual cultures. Data are shown as the mean ± SEM.