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. 2022 Jan 12;13:290. doi: 10.1038/s41467-021-27915-z

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — A Top panel: Capacitance of CHO cells transiently infected with Slc26a9, scaled to linear capacitance near −100 mV. Measured with dual-sine voltage superimposed on a voltage ramp from −175 to +150 mV. A very slight increase in Cm occurs at +150 mV, possibly indicating an extremely right-shifted NLC. Capacitance data are presented as mean values (± SEM) of 10 independent cells. Inset: Confocal image of CHO cells transfected with Slc26a9-YFP that is expressed on the membrane. The scale bar is 10 microns. Bottom panel: Ramp induced currents simultaneously measured with membrane capacitance, also scaled to linear capacitance. Current data are presented as mean values (± SEM) from the same 10 independent cells shown in the upper panel. Average (± SEM) series and membrane resistance are shown. In red are mean (±SEM) currents observed in untransfected cells (n = 5). Inset: top traces show very small nonlinear currents extracted with P/−5 protocol, subtraction holding potential set to −50 mV. Voltage protocol shown below traces. B Top panel: Capacitance of CHO cells transiently infected with prestin, scaled to linear capacitance near +100 mV. Measured with dual-sine voltage superimposed on a voltage ramp from −175 to +150 mV. A prominent increase in Cm occurs at −110 mV, typical of prestin NLC. Capacitance data are presented as mean values (± SEM) of nine independent cells. Bottom panel: Ramp-induced currents simultaneously measured with membrane capacitance, also scaled to linear capacitance. Current data are presented as mean values (± SEM) from the same nine independent cells shown in the upper panel. Average (± SEM) series and membrane resistance are shown. In red are mean (± SEM) currents observed in untransfected cells (n = 5). Inset: top traces show large nonlinear displacement currents extracted with P/-5 protocol, subtraction holding potential set to +50 mV. Voltage protocol (holding potential 0 mV) shown below traces. Source data are provided as a Source Data file.