Table 4.
The yield (ng) of enriched DNA library pools for different protocols.
Pool ID | Protocol | Baits | input DNA lib, ng | PCR kit | Cycles post-PCR | output DNA lib, ng |
---|---|---|---|---|---|---|
1A | RSMU_exome | Agilent v6 | 4800 | KAPA | 7 (½ volume) | 139 |
1B | RSMU_exome | Agilent v6 | 4800 | KAPA | 7 (½ volume) | 144 |
2A | RSMU_exome | MGI v4 | 4800 | KAPA | 10 (½ volume) | 520 |
2B | RSMU_exome | MGI v4 | 4800 | KAPA | 10 (½ volume) | 585 |
3A | MGI | MGI v4 | 2000 | MGI | 13 | 127 |
3B | MGI | MGI v4 | 2000 | MGI | 13 | 123 |