Fig. 1. SMYD2 is highly expressed in CRC and SMYD2 deficiency reduces colon tumor growth in vivo.

Immunofluorescence staining of murine Apc^Min/+ (A) or human (B) colon tumor and tumor-free tissues with anti-SMYD2 antibody. Hoechst was used to stain the nuclei. C Immunoblot analysis of SMYD2 in lysates of human or murine colon tumor and tumor-free tissues. β-Actin served as a loading control. D, E WT and Smyd2 CRISPR/Cas9 knockout MC-38 cells were implanted into mice via submucosal injection. D Representative images of the colonic tumors and miniendoscopy-assisted tumor scoring. E Measurements of colonic tumor volume upon euthanasia. F Tumor volume from mice in which CRISPR/Cas9-directed SMYD2 knockout or WT HT-29 cells were injected subcutaneously and G tumors weight at day 20 from mice shown in (F) above.