Fig. 5. Inhibition of SMYD2 with AZ505 or BAY-598 suppressed colon tumor growth.

A−C Apc^Min/+ tumors were intratumorally injected with AZ505 or DMSO every two days. A, B Tumor size scored using colonoscopy at indicated time points (n = 5/group). C Representative images of H&E and cleaved-caspase3 (red) stained Apc^Min/+ tumor sections (scale bar, 25 µm). The graph shows quantification of cleaved-caspase3 positive cells in tumor tissues. D, E HT-29 cells were injected subcutaneously into Rag1^−/− mice. Once tumor size was measureable, mice were intraperitoneal injected with BAY-598 or DMSO daily (n = 6/group). D Relative tumor volume monitored using calipers at three-day intervals. Tumor volume was normalized to the volume at the beginning of treatment. E Tumor weight at day 10 post euthanasia. F Representative images of H&E and cleaved-caspase3 (red) stained tumor sections (scale bar, 25 µm). The graph shows quantification of cleaved-caspase3 positive cells in tumor tissues.