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. 2022 Jan 12;12:638. doi: 10.1038/s41598-021-04705-7

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Neutrophil and monocyte subpopulations are disturbed in COVID-19 patients. (A–H) Percentages of positive cells and surface expression of markers related to subpopulations in neutrophils by flow cytometry. (A) LOX-1 + neutrophils, (B), PD-L1 + neutrophils, (C) ILT3 + CD62L + neutrophils, (D) CD13^low neutrophils, (E) CD10 expression on neutrophils, (F) CD11b^lowCD16^low neutrophils, (G) CD49 + neutrophils, (H) HLA-DR expression on neutrophils. (I–K) Percentages of monocytes subpopulations by flow cytometry. (I) CD14 + CD16- classical monocytes, (J) CD14 + CD16 + intermediate monocytes, (K) CD14^low/-CD16 + atypical monocytes. Intergroup comparison by Mann–Whitney U test between healthy controls and ICU or non-ICU patients: ****P < 0.0001, ***P < 0.001, **P < 0.01, *P < 0.05, between non-ICU and ICU patients: ^####P < 0.0001, ^###P < 0.001, ^##P < 0.01, ^#P < .05. All boxplots whiskers represent 10th and 90th percentiles. HC healthy controls.