Table 1.
TAP-MS data for AtEPL1B combined with metaanalysis of the previously published AP-MS experiments using NuA4 subunits as baits.
| Sc name | At gene | At name | AtARP4 | AtARP4 | AtSWC4 | HAM1 | HAM2 | AtEPL1A | AtEPL1B | AtYAF9A | AtEPL1B | |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Complexes | – | – | – | NuA4, SWR1, SWI/SNF, INO80 | NuA4, SWR1, SWI/SNF, INO80 | NuA4, SWR1 | NuA4, PEAT | NuA4, PEAT | NuA4 | NuA4 | NuA4, SWR1 | NuA4 |
| Model | – | – | – | Cell culture | Cell culture | Cell culture | Seedlings | Seedlings | Seedlings | Seedlings | Seedlings | |
| Promoter | – | – | – | CaMV35S | UBQ10 | UBQ10 | Native | Native | Native | Native | CaMV35S | Native |
| Tag | – | – | – | GS TAP tag | Strep | Strep | Myc | Myc | Flag | Flag | GH TAP tag | GS TAP tag |
| Reference | – | – | – | Vercruyssen | Bieluszewski | Bieluszewski | Tan | Tan | Tan | Tan | Crevillen | This work |
| Conserved NuA4 subunits | ARP4 | AT1G18450 | AtARP4 | + | + | + | + | + | + | + | + | 2/1.78 |
| AT1G73910 | AtARP4A | – | + | + | – | – | – | – | – | – | ||
| SWC4 | AT2G47210 | AtSWC4 | – | + | + | + | – | + | + | + | 2/1.63 | |
| YAF9 | AT5G45600 | AtYAF9A | + | + | + | + | + | + | + | + | 2/1.42 | |
| AT2G18000 | AtYAF9B | – | – | + | – | – | – | + | – | – | ||
| EAF1 | AT3G24880 | AtEAF1A | – | + | + | + | + | + | + | + | 2/0.42 | |
| AT3G24870 | AtEAF1B | – | + | + | + | – | – | – | – | 2/0.43 | ||
| TRA1 | AT2G17930 | AtTRA1A | – | – | – | – | – | + | + | – | 2/0.37 | |
| AT4G36080 | AtTRA1B | – | – | + | – | + | + | + | – | 2/0.44 | ||
| ESA1 | AT5G64610 | HAM1 | – | + | + | + | – | + | + | + | 2/0.95 | |
| AT5G09740 | HAM2 | – | – | + | – | + | – | – | – | 1/0.16 | ||
| EPL1 | AT1G16690 | AtEPL1A | – | – | + | + | – | + | – | – | – | |
| AT1G79020 | AtEPL1B | – | + | + | – | + | – | + | – | 2/2.22 | ||
| YNG2 | AT1G54390 | AtING2 | – | + | + | + | – | + | + | – | 2/1.50 | |
| EAF6 | AT4G14385 | AtEAF6 | – | + | + | + | – | + | + | – | 2/1.96 | |
| EAF3 | AT4G37280 | MRG1 | – | – | + | + | – | – | – | – | 2/0.29 | |
| AT1G02740 | MRG2 | – | – | – | – | – | – | – | – | – | ||
| EAF5 | – | – | – | – | – | – | – | – | – | – | – | |
| EAF7 | AT1G26470 | AtEAF7 | – | – | + | – | – | – | – | – | – | |
| Additional AtEPL1B interactors detected here | AT2G31810 | AHASS1 | – | – | – | – | – | – | – | – | 2/0.21 | |
| AT3G07810 | AOG2 | – | – | – | + | – | – | – | – | 2/0.18 | ||
| AT3G60110 | BRD6 | – | – | + | + | – | – | – | – | 2/0.06 | ||
| AT1G48520 | GATB | – | – | – | – | – | – | – | – | 1/0.06 | ||
| AT1G26570 | UGD1 | – | – | – | – | – | – | – | – | 1/0.04 | ||
| Number of additional bait-specific hits | 2 | 454 | 1104 | 331 | 280 | 96 | 241 | 114 | 0 | |||
NSAF normalized spectral abundance factor.
Numbers in the rightmost column indicate the number of biological replicates (out of two) in which the protein was detected as well as its relative abundance in the final eluate analyzed by mass spectrometry.
Values in bold indicate proteins for which peptides were detected in both biological replicates.