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. 2021 Apr 5;54(1):157–173. doi: 10.4143/crt.2020.1063

Fig. 3.

Fig. 3

Effects of docetaxel (DOC) and Ku-0063794 (KU), either individually or in combination, on epithelial-mesenchymal transition (EMT) and migration of breast cancer cells. (A) Western blot analyses showing the expression of EMT-related markers in MCF-7 breast cancer cells following mono- and combination therapies of DOC and KU. Relative densities of individual markers. The relative densities had been quantified using Image J software and then were normalized to the density of β-actin in each group. (B) Western blot analyses showing the expression of EMT-related markers in MCF-7 and MDA-MB-231 triple-negative breast cancer (TNBC) cells following mono- and combination therapies of DOC and KU. Relative densities of individual markers. (C) Wound healing assay (×200, scale bar=20 μM) showing the effects of DOC and KU, either individually or in combination, on the migration of MCF-7 and MDA-MB-231 breast cancer cells. Migration was expressed as percentage of cells migrated compared to the control. Values are presented as mean±standard deviation of three independent experiments. *p < 0.05.