Table 1.
The result of NGS and Sanger sequencing for TERT promoter area
| Case No. | Surgical diagnosis | WHO grade | NGS TERT promoter (initial) | Sanger sequencing | NGS TERT promoter (after reviewing IGV) | TERT TD | TERT VC | TERT VAF (%) |
|---|---|---|---|---|---|---|---|---|
| 1 | Glioblastoma, IDH-wildtype | IV | Wild type | Not detected | Wild type | 13 | 0 | 0 |
| 2 | Glioblastoma, IDH-wildtype | IV | Wild type | c.1-146C>T (C250T) | c.1-146C>T (C250T) | 7 | 2 | 28.6 |
| 3 | Glioblastoma, IDH-wildtype | IV | Wild type | c.1-124C>T (C228T) | c.1-124C>T (C228T) | 8 | 6 | 75 |
| 4 | Glioblastoma, IDH-wildtype | IV | Wild type | Not detected | Wild type | 9 | 0 | 0 |
| 5 | Glioblastoma, IDH-wildtype | IV | Wild type | c.1-124C>T (C228T) | c.1-124C>T (C228T) | 16 | 7 | 43.8 |
| 6 | Glioblastoma, IDH-wildtype | IV | Wild type | c.1-124C>T (C228T) | c.1-124C>T (C228T) | 25 | 5 | 20 |
| 7 | Glioblastoma, IDH-wildtype | IV | Wild type | c.1-124C>T (C228T) | Wild type | 2 | 0 | 0 |
| 8 | Glioblastoma, IDH-wildtype | IV | Wild type | c.1-124C>T (C228T) | c.1-124C>T (C228T) | 9 | 2 | 22.2 |
| 9 | Glioblastoma, IDH-wildtype | IV | Wild type | c.1-124C>T (C228T) | c.1-124C>T (C228T) | 14 | 2 | 14.3 |
| 10 | Glioblastoma, IDH-wildtype | IV | Wild type | c.1-146C>T (C250T) | c.1-146C>T (C250T) | 7 | 3 | 42.9 |
| 11 | Glioblastoma, IDH-wildtype | IV | Wild type | Not detected | Wild type | 24 | 0 | 0 |
| 12 | Glioblastoma, IDH-wildtype | IV | Wild type | Not detected | Wild type | 26 | 0 | 0 |
| 13 | Glioblastoma, IDH-wildtype | IV | Wild type | Not detected | Wild type | 25 | 0 | 0 |
| 14 | Glioblastoma, IDH-wildtype | IV | Wild type | c.1-146C>T (C250T) | c.1-146C>T (C250T) | 28 | 6 | 21.4 |
| 15 | Glioblastoma, IDH-wildtype | IV | Wild type | Not detected | Wild type | 17 | 0 | 0 |
| 16 | Glioblastoma, IDH-wildtype | IV | Wild type | c.1-146C>T (C250T) | c.1-146C>T (C250T) | 27 | 13 | 48.2 |
| 17 | Glioblastoma, IDH-wildtype | IV | Wild type | Not detected | Wild type | 28 | 0 | 0 |
| 18 | Glioblastoma, IDH-wildtype | IV | Wild type | c.1-146C>T (C250T) | c.1-146C>T (C250T) | 37 | 10 | 27 |
| 19 | Glioblastoma, IDH-wildtype | IV | Wild type | c.1-146C>T (C250T) | c.1-146C>T (C250T) | 32 | 11 | 34.4 |
| 20 | Glioblastoma, IDH-wildtype | IV | Wild type | c.1-124C>T (C228T) | c.1-124C>T (C228T) | 40 | 6 | 15 |
| 21 | Glioblastoma, IDH-wildtype | IV | Wild type | c.1-124C>T (C228T) | c.1-124C>T (C228T) | 47 | 28 | 59.6 |
| 22 | Glioblastoma, IDH-wildtype | IV | Wild type | c.1-146C>T (C250T) | c.1-146C>T (C250T) | 30 | 6 | 20 |
| 23 | Glioblastoma, IDH-wildtype | IV | Wild type | c.1-124C>T (C228T) | c.1-124C>T (C228T) | 12 | 4 | 33.3 |
| 24 | Glioblastoma, IDH-wildtype | IV | Wild type | c.1-124C>T (C228T) | c.1-124C>T (C228T) | 26 | 11 | 42.3 |
| 25 | Glioblastoma, IDH-wildtype | IV | Wild type | Not detected | Wild type | 28 | 0 | 0 |
IDH, isocitrate dehydrogenase; IGV, integrative genomics viewer; NGS, next-generation sequencing; TD, total read depth; TERT, telomerase reverse transcriptase; VAF, variant allele frequency; VC, variant read count; WHO, World Health Organization.