Fig. 5.

Analysis of Raw 264.7 cell culture and differentiation on 15 mM Fmoc-FF/S and Fmoc-FF/S/RGD hydrogels with and without incorporated Hap. (A) F-actin staining of Raw 264.7 cells after seven days culture on the different hydrogels (green: F-actin, Alexa Fluor 488 phalloidin; blue: Nuclei, Hoechst 33342; scale bars 20 µm). (B) Measurements of cell diameter (data shown as mean ± SD, N=43, **p <0.05). (C) Analysis of multinucleation by a violin distribution plot (data shown as number of nuclei per cell, N=100). (D) SEM images of Raw 264.7 cells cultured on Fmoc-FF/S/RGD with or without incorporated Hap. White arrow shows elongated pseudopodia of Raw 264.7-derived OC. (Data are shown as mean ± SD; ***p-value <0.001; **p-value <0.05).