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. 2021 Dec;9(24):1770. doi: 10.21037/atm-21-6134

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2021 Annals of Translational Medicine. All rights reserved.

Open Access Statement: This is an Open Access article distributed in accordance with the Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License (CC BY-NC-ND 4.0), which permits the non-commercial replication and distribution of the article with the strict proviso that no changes or edits are made and the original work is properly cited (including links to both the formal publication through the relevant DOI and the license). See: https://creativecommons.org/licenses/by-nc-nd/4.0.

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The lncRNA GAS5 sponges miR-96-5p by direct targeting. (A) Heatmap showing the hierarchical clustering of differentially-expressed miRNAs in patients and normal controls. Red: up-regulated genes; green: down-regulated genes. (B) The FISH assay revealed that lnc-GAS5 was localized in the cytoplasm and the nucleus of SKOV3 cells. (C) MiR-96-5p expression was detected by real-time qPCR in HEY and SKOV3 cell lines transfected with LV-GAS5 and NC. (D) The miR-96-5p binding site in lnc-GAS5 3' UTR. The binding relationship between GAS5 and miR-96-5p was detected using the luciferase reporter assay with NC or Mut luciferase reporter plasmids of GAS5. *, P<0.05; ***, P<0.001. lncRNA, long non-coding RNA; GAS5, growth arrest-specific transcript 5; FISH, fluorescence in situ hybridization; qPCR, quantitative reverse transcription polymerase chain reaction.