Figure 2.
Rg1 reduced HG-induced apoptosis of HBZY-1 cells. HBZY-1 cells were induced by HG (30 mmol/L). HBZY-1 cells were divided into 5 groups: control group (HBZY-1 cells without any treatment); HG group (HBZY-1 cells were induced by 30 mmol/L glucose); HG + Rg1 (2.5, 5, 10 µmol/L) group, where HG-induced HBZY-1 cells were treated with 2.5, 5, and 10 µmol/L Rg1 at 37 °C for 48 h. (A,B) The apoptosis rate of HBZY-1 cells was detected by the flow cytometry assay. (C) Levels of Bcl-2, Bax, cleaved caspase-3, and cleaved caspase-9 were measured by western blot. *P<0.05 versus control; #P<0.05, ##P<0.01 versus the HG group. Rg1, ginsenoside Rg1; HG, high glucose.