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. 2021 Dec 30;12:773340. doi: 10.3389/fendo.2021.773340

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Copyright © 2021 Long, Liu, Gao, Ni, Qian, Ni, Fang, Jia and Li

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effect of B. adolescentis on intestinal permeability. Mice were grouped and treated as in Figure 1 . (A, B) Abundance of B.adolescentis in feces and mucosa after intervention determined by qPCR (n = 6 in each group). (C) Serum concentrations of DX-4000-FITC at 1 hour after oral gavage (n = 6 in each group). (D) ZO-1and occludin and in ileum detected by immunofluorescent staining. (E, F) H-score of ZO-1 and occludin measured in ileum section with immunofluorescent staining. (G) The level of serum LPS (n = 6 in each group). DX-4000-FITC, fluorescent-labeled dextran; H-score, histochemistry score; LPS, lipopolysaccharide; ZO-1, zonula occludens-1. Data are presented as mean ± SEM. Significance was determined by one-way ANOVA with Fisher’s LSD multiple-comparison analysis. *p < 0.05; **p < 0.01; ***p < 0.001.