FIGURE 1.
Library preparation for rapid nanopore sequencing. The workflow includes a standard ~2.5-hour SurePlex whole genome amplification, ~18 minute rapid end-preparation and purification, a 1-step barcode and sequencing adapter ligation and purification, and a 20-minute to 2-hour nanopore sequencing run, depending on the number of samples multiplexed into a single run. Components used in library preparation were specified in colors (gray, DNA fragments; red, native barcoding adapter; blue and orange, 1D sequencing adapter).