Figure 3.

miR-15a inhibits the proliferation of prostate cancer cells by regulating GPX4. (A) Reverse transcription-quantitative PCR analysis was performed to detect GPX4 mRNA expression in LNCAP cells transfected with or without si-GPX4. LNCAP cells were transfected with miR-15a mimics, si-GPX4 or NCs and the (B) Cell Counting Kit-8 assay was performed to assess cell proliferation and the (C) inhibitory ratio. (D) LDH levels in the supernatant of cultured cells were measured. The experiments were performed in triplicate. **P<0.01 vs. control or si-NC group. miR, microRNA; GPX4, glutathione peroxidase 4; si, small interfering; NC, negative control; LDH, lactate dehydrogenase; OD, optical density.