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. 2021 Dec 14;23(2):142. doi: 10.3892/etm.2021.11065

Figure 2.

Figure 2

miR-126 overexpression negatively regulates PIK3R2 expression in EPCs. (A) Possible binding sites between miR-126 and PIK3R2 3'UTR. (B) 293T cells were transfected with the mimic control or miR-126 mimic for 48 h, before miR-126 expression was detected using RT-qPCR. (C) Potential interactions between miR-126 and PIK3R2 were assessed using dual-luciferase reporter assay. (D-H) EPCs were transfected with control-plasmid, PIK3R2-plasmid, mimic control, miR-126 mimic, miR-126 mimic + control-plasmid or miR-126 mimic+PIK3R2-plasmid under hypoxic conditions. (D) PIK3R2 and (E) miR-126 mRNA expression in EPCs was measured by RT-qPCR after plasmid or mimic transfection, respectively. (F) PIK3R2 mRNA expression in EPCs was measured by RT-qPCR after mimic and plasmid co-transfection. (G) PIK3R2 protein expression in EPCs was measured by western blot analysis, (H) which was quantified. **P<0.01 vs. mimic control. ##P<0.01 vs. control-plasmid. &&P<0.01 vs. miR-126 mimic + control-plasmid. miR, microRNA; UTR, untranslated region; WT, wild-type; MUT, mutant; RT-qPCR, reverse transcription-quantitative PCR; EPCs, endothelial progenitor cells; PIK3R2, PI3K regulation subunit 2.