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. 2021 Dec 14;23(2):142. doi: 10.3892/etm.2021.11065

Figure 6.

Figure 6

Effects of VEGF and miR-126 mimic transfection on the viability, tube-forming ability and migration of EPCs under hypoxic conditions. EPCs were transfected with control-plasmid, VEGF-plasmid, VEGF-plasmid + mimic control or VEGF-plasmid + miR-126 mimic under hypoxic conditions for 72 h. (A) MTT assay was used to evaluate the cell viability of transfected EPCs. (B) Tube formation assay was performed to measure the tube-forming ability of transfected EPCs. Magnification, x100. (C) Tube formation assay was quantified. (D) Transwell assay was used to measure the migration ability of transfected EPCs Magnification, x200. (E) Transwell assay was quantified. **P<0.01 vs. hypoxia + control-plasmid. ##P<0.01 vs. hypoxia + VEGF-plasmid + mimic control. miR, microRNA; EPCs, endothelial progenitor cells.