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. 2021 Dec 14;23(2):142. doi: 10.3892/etm.2021.11065

Figure 7.

Figure 7

Effects of VEGF and miR-126 mimic transfection on the PI3K/AKT pathway in EPCs under hypoxic conditions. EPCs were transfected with control-plasmid, VEGF-plasmid, VEGF-plasmid + mimic control or VEGF-plasmid + miR-126 mimic under hypoxic conditions for 72 h. (A) Western blot analysis was used to detect the protein levels of p-AKT and AKT in transfected EPCs. (B) p-AKT/AKT ratio was quantified. (C) Reverse transcription-quantitative PCR analysis was used to measure the mRNA expression of AKT in transfected EPCs. **P<0.01 vs. hypoxia + control-plasmid. ##P<0.01 vs. hypoxia + VEGF-plasmid + mimic control group. EPCs, endothelial progenitor cells; miR, microRNA; p-, phosphorylated.