Figure 3.

TRPM8 expression in the brain cortex tissue and in cerebral vascular cells. Cerebral microvessels (60–300 µm) and the astrocyte-enriched fraction of the brain parenchyma were obtained from the newborn pig brain cortex. Cerebral vascular endothelial and smooth muscle cells and cortical astrocytes were grown in primary cultures (N = 4 independent experiments). (a) Representative blots of TRPM8 expressed in the brain cortex tissue and in neurovascular cells. (b) TRPM8 expression normalized to housekeeping gene actin; Values are means ± SD.