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. 2021 May 5;2(3):100034. doi: 10.1016/j.xhgg.2021.100034

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© 2021 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

PMC Copyright notice

LSM7 mRNA and protein levels are decreased in individual 1 compared to control individuals

(A) RT-qPCR analysis of LSM7 mRNA extracted from individual 1’s fibroblasts and two age- and sex-matched control individuals (n = 6). The results are represented in terms of fold change after normalizing to RPL30 and SDHA mRNAs. Each value represents the mean ± SEM (unpaired t test [two tailed] ∗∗p < 0.01).

(B) Total protein lysates extracted from individual 1 and age- and sex-matched control individual fibroblasts were immunoblotted with anti-LSM7 and anti-beta-tubulin.

(C) The pixel densities of immunoblot bands from at least 3 independent replicates were quantified using ImageJ software, normalized over beta-tubulin immunoblot and represented as mean ± SEM (unpaired t test [two tailed], ∗∗∗p < 0.001).