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. 2021 Dec 27;23(2):63. doi: 10.3892/ol.2021.13181

Figure 1.

Figure 1.

PKMYT1 is upregulated in SCC-9 cells, and its knockdown inhibits cell proliferation. (A) PKMYT1 protein expression was detected using western blot analysis. (B) PKMYT1 mRNA expression was detected using RT-qPCR. (C and D) Relative protein and mRNA expression levels were detected using (C) western blot analysis and (D) RT-qPCR, respectively. Cell proliferation was detected using (E) Cell Counting Kit-8. *P<0.05, **P<0.01, ***P<0.001 vs. sh-NC. (F) Cell colony formation was determined using colony formation assays. **P<0.01, ***P<0.001. PKMYT1, protein kinase, membrane-associated tyrosine/threonine 1; RT-qPCR, reverse transcription-quantitative PCR; sh-NC, negative control short hairpin RNA.