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. 2021 Dec 27;23(2):63. doi: 10.3892/ol.2021.13181

Figure 3.

Figure 3.

PKMYT1 knockdown inhibits the proliferation of SCC-9 cells by regulating CCNA2 expression. (A) PKMYT1 was found to be able to bind CCNA2 according to the STRING and GeneMANIA databases. (B and C) Relative mRNA and protein expression levels were detected using (B) RT-qPCR and (C) western blot analysis, respectively. (D) Co-IP assay was used for verification of the binding between PKMYT1 and CCNA2. (E) CCNA2 expression in the sh-PKMYT1 group was detected using western blot analysis. (F) CCNA2 expression in the pcDNA-CCNA2 group was detected using western blot analysis. (G) A Cell Counting Kit-8 assay was used for the determination of cell proliferation. *P<0.05 vs. sh-PKMYT1 + pcDNA. (H) A colony formation assay was used for the detection of cell colony formation. **P<0.01, ***P<0.001. PKMYT1, protein kinase, membrane-associated tyrosine/threonine 1; CCNA2, cyclin A2; RT-qPCR, reverse transcription-quantitative PCR; co-IP, Co-immunoprecipitation; sh, short hairpin; sh-NC, negative control short hairpin RNA; WB, western blotting.