Figure 3.

NirS gene neighborhood in SURF MAG 42 versus Pseudomonas aeruginosa. Gene neighborhood analyses of the 20,000 base pair region surrounding nirS differ markedly between P. aeruginosa PAO1 (GenBank reference sequence NP_249210.1, top) and two Chloroflexi genomes containing the C1‐C2‐NirS gene: SURF MAG 42 (center) and Anaerolinea thermolimosa (bottom). While the P. aeruginosa nitrite reductase occurs as part of a larger nir operon, and in close proximity to nitric oxide reductase genes, the nirS ORF neighborhoods in SURF MAG 42 and A. thermolimosa do not appear to contain other denitrification‐specific genes. Detailed descriptions of ORF/gene families and functions can be found in Table A1. MAG, metagenome‐assembled genome; ORF, open reading frame; SURF, Sanford Underground Research Facility