Figure A4.

Subsampled C2 domain tree. Phylogenetic analysis of C2 domain homologs, subsampled to contain only taxa with both C2 and NirS domains in the nitrite reductase ORF, places the largest clade of Chloroflexi as sister to a polyphyletic group including a large group of Alpha‐, Beta‐, and Gammaproteobacteria. Support values for selected bipartitions are labeled (aLRT/bb). Support for other nodes is indicated with the following color scheme: Strong support with both values ≥90 (black); weak support with both values ≤50 (white); intermediate support with one or both values between 50 and 90 (gray); conflicting support, with one value ≤50 and the other ≥90 (gray). ORF, open reading frame