Fig. 3.

Sequential transcytosis of caveolae-specific antibodies across the vascular endothelium in vivo.

(A) Electron micrographs showing the gold particle labeled mAPP2 antibody entering and targeting luminal caveolae within 2–3 min after perfusion through isolated lungs.

(B) Transcytosis with clear endothelial release of gold particles from abluminal caveolae into the underlying perivascular space and lung interstitium.

(C) Gold particles taken up by epithelial caveolae, transcytosed across the epithelium, and released into airways. (Reprinted with permission from Ref. [36]. Copyright 2002 National Academy of Sciences [Proceedings of the National Academy of Sciences of the USA]).