TABLE 1.
Comparison of three HIV-1 detection assays
| Patient no. | HIV-1 RNA level in plasma (copies/ml) | Presence of HIV-1 p24 determined with MAb(s) and input cellsa
|
ACTG PBMC culture resulte with 10 × 106 PBMC | ||
|---|---|---|---|---|---|
| Anti-CD3-CD8b and 10 × 106 PBMC | Anti-CD3-CD8b and 1 × 106 PBMC | Anti-CD3 and anti-CD28c and 2 × 106 CD8− cells | |||
| 1 | 30,000 | + (7) | + (7) | + (7) | + (7) |
| 2 | 29,930 | + (7) | + (7) | + (7) | + (7) |
| 3 | 2,415 | + (7) | − | + (7) | − |
| 4 | 1,440 | + (7) | + (7) | + (7) | + (7) |
| 5 | <200 | + (14) | + (7) | NDd | + (22) |
| 6 | <200 | + (14) | − | + (7) | − |
| 7 | <200 | + (14) | − | + (22) | + (7) |
| 8 | <200 | + (14) | + (14) | + (22) | − |
| 9 | <200 | + (14) | + (14) | ND | − |
| 10 | <200 | + (20) | − | − | − |
| 11 | <200 | + (22) | − | − | − |
a
Detected by enzyme-linked immunosorbent assay. Values in parentheses indicate the number of days after the start of culture. Cultures ended at 21 days.
b
CD8 T-cell depletion and CD4 cell stimulation were achieved by the bispecific MAb CD3-CD8.
c
CD8 T-cell depletion and CD4 cell stimulation were achieved by immobilized anti-CD3 and anti-CD28 MAbs.
d
ND, not done.
e
Differences between CD3-CD8 and ACTG cultures were significant (P = 0.02, Fisher's exact test). Values in parentheses indicate the number of days after the start of culture.