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. Author manuscript; available in PMC: 2022 Mar 28.
Published in final edited form as: Sci Signal. 2021 Sep 28;14(702):eabf6584. doi: 10.1126/scisignal.abf6584

Fig. 1. Experimental design.

Fig. 1.

Cecal, colonic, and fecal samples from isogenic controls WT and Rag mice, as well as the colitic RagT group (6 mice per group, 18 total) were collected on day 56 for metabolome extraction and liquid chromatography-mass spectrometry untargeted metabolomics analysis. Following data analysis, features were prioritized for MS/MS experiments, metabolite identification, and differential analysis to find altered levels of molecules in RagT colitis samples. A panel of metabolites with significantly altered levels in IBD, and no prior known role in this disease, were profiled in cell-based assays that can reveal pro-inflammatory and anti-inflammatory properties.