Table II.

Metabolic fluxes determined from labeling experiments

	Flux	MCL-RL control	MCL-RL IBR	JeKo-1 control	JeKo-1 IBR
f1	Fmrg. metabolic rate of glucose. Glucose → G-6-P	246±19	55±4	199±5	98±4
f2*	Fppp. 6-P-G → Ru-5-P	31.6±12.9	5.0±2.0	15.4±6.3	8.1±3.3
f3*	Ftk1. Ri-5-P + X-5-P → GA-3-P + S-7-P	10.5±4.3	1.7±0.7	5.2±2.1	2.7±1.1
f4*	Ftk2. X-5-P + E-4-P → F-6-P + Ga-3-P	10.5±4.3	1.7±0.7	5.2±2.1	2.7±1.1
f5*	Fta. GA-3-P + S-7-P → F-6-P + E-4-P	10.5±4.3	1.7±0.7	5.2±2.1	2.7±1.1
f12**	Fpdh. pyruvate → acetyl-CoA	31.5±6.0	12.9±2.4	19.4±3.7	15.8±3.0
f13**	Fcs. acetyl-CoA → citrate	31.5±6.0	12.9±2.4	19.4±3.7	15.8±3.0
f16**	Fgls. glutaminei → glutamate	17.0±2.9	4.6±0.8	5.9±1.0	5.8±1.0
f12***	Fpdh. pyruvate → acetyl-CoA	33.3±5.0	17.5±2.7	40.6±6.1	31.1±5.1
f13***	Fcs. acetyl-CoA → citrate	33.3±5.0	17.5±2.7	40.6±6.1	31.1±5.1
f14***	Fidh3. citrate → isocitrate (mitochondrial)	6.4±0.9	4.7±0.8	4.4±0.8	3.5±0.5
f16***	Fgls. glutaminemito → glutamatemito	27.0±5.0	12.7±2.5	36.2±6.8	27.6±5.0
f22+f23***	Frc. reductive carboxylation. αKG → isocitrate (cytosolic plus mitochondrial)	4.3±0.7	3.1±0.4	5.5±0.8	3.6±0.6
f30***	Fkgdh. α-KG →succinate/SucCoA	33.3±5.0	17.5±2.9	40.6±5.4	31.1±4.7
f31***	Fsdh. succinate/SucCoA → fumarate	33.3±5.0	17.5±2.9	40.6±5.4	31.1±4.7
f32***	Ffuma. fumarate → malate	33.3±5.0	17.5±2.9	40.6±5.4	31.1±4.7
f33***	Fmdh. malate →OAA	33.3±5.0	17.5±2.9	40.6±5.4	31.1±4.7
***	ATPcyto	468±51	105±12	379±42	187±21
***	ATPmito	485±68	254±36	590±83	452±63
***	ATPq	270±35	128±17	362±47	277±36
***	%LIPdnQ	14±3	20±4	13±3	12±3
***	WE	12.8±2.5	4.4±0.9	8.3±1.8	5.4±1.1
***	%ANAq	98±21	96±21	99±21	98±20

^*Determined from the [1,2-¹³C₂] glucose labeling experiment. Unit = nmole/10⁶ cell/h.

^**Determined from the [1,6-¹³C₂] glucose labeling experiment; relative to F_idh3 flux in the TCA cycle arbitrarily set to 10.

^***Determined from the [U-¹³C₅] glutamine labeling experiment. Unit = nmole/10⁶ cell/h for fluxes and ATP production rates.

ATP_cyto, cytosolic ATP production rate

ATP_mito, mitochondrial ATP production rate

ATP_q, ATP production rate from glutamine

%LIPdnQ, glutamine contribution (%) to de novo lipid synthesis

WE, $\text{Warburg}\text{effect}\text{parameter}=\frac{\text{Pyruvate}\to \text{Lactate}}{\text{Pyruvate}\to \text{mitochondria}}$

%ANA_q, glutamine contribution (%) to total anaplerosis