Figure 1. Testosterone immunoreactivity after secretome injection in testicular dysfunction rats treated with secretome. The reactivity of testosterone was detected in the interstitial cells of the testis (red arrows). Immunoreactivity of testosterone was not detected 1 week after the first secretome injection in the low dosage group (A) and high dosage group (B). One week after the second secretome injection, in the low dosage group (C), the immunoreactivity of testosterone was moderate; meanwhile, in the high dosage group (D), the immunoreactivity of testosterone was strong. One week after the third secretome injection, in the low dosage (E) and high dosage group (F), the immunoreactivity of testosterone was strong. One week after the fourth secretome injection, in the high dosage group (G), the immunoreactivity of testosterone was strong. There was strong reactivity of testosterone in Leydig cells with 40× magnification (H).