Table 1.
SEC-MALS Mwt summary.
| Material | Mw (kDa) | % protein | % polysaccharide |
|---|---|---|---|
| Native PGPS | 30,315 | n/a | n/a |
| Sized PGPS | 270 | n/a | n/a |
| Sized PGPS eCRM Conjugate | 2,185 | 51.2 | 48.7 |
| dn/dc PS = 0.133 mL/g; dn/dc Protein = 0.185 mL/g; | |||
| Ext.Coeff.eCRM = 0.9 mL/(mg cm); Ext.Coeff.PS = 0.0 mL/(mg cm) | |||
Molecular weight measurements of the P. gingivalis CPS at the various stages in the production of the final vaccine. The relative composition of polysaccharide and protein in the final conjugate product is based on the differential detector responses in the dRI and UV, as described in the text.