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. 2020 Dec 3;2:604160. doi: 10.3389/fmedt.2020.604160

Figure 1.

Figure 1

Expression of the recombinant ZIKV NS1 and the chimeric ZIKV NS1 and HSV-1 gD. (A,B) Schematic representations of pNS1-ZIKV, encoding the full length ZIKV NS1 protein (A) and pgDNS1-ZIKV, encoding the genetic fusion of NS1 and gD (B). (C–E) Detection of the ZIKV NS1 expressed in HEK293-T cells 24 h after transfection with the tested plasmids. Cells were labeled with anti-ZIKV NS1 mAb followed by anti-mouse IgG antibodies conjugated to Alexa Fluor 488 and analyzed by flow cytometry (C,D) and immunofluorescence (E). Data are expressed as mean ± SEM of two independent experiments performed in duplicate. Scale bar = 400 μm. Statistical significance was calculated using One-way ANOVA with post-test Bonferroni (**p < 0.01, ****p < 0.0001, NS = not significant).