Figure 6.

Film autoradiogram of 20 μm coronal sections of rhesus monkey brain removed 120 min after IV injection of the HIRMAb-IDS fusion protein (A) or IDS enzyme (B). The forebrain section is on the top, the midbrain section is in the middle, and the hindbrain section with cerebellum is on the bottom. Scans produced after labeling of the HIRMAb-IDS fusion protein or IDS with the [¹²⁵I]-Bolton-Hunter reagent. Xrays exposed for 7 days. HIRMAb, human insulin receptor monoclonal antibody; IDS, iduronate 2-sulfatase. Fusion of the IDS enzyme, which alone does not cross the BBB (B), to the HIRMAb enables global penetration of the lysosomal enzyme throughout the primate brain (A). Reproduced with permission from Boado et al. (142).