Table 2.

Organ enrichment of NPC1 mRNA, relative to GAPDH mRNA, in THL treated NPC1 mice as compared to vehicle treated NPC1 mice.

Organ	Treatment	NPC1 Cq	GAPDH Cq	ΔCq	ΔΔCq	NPC1 mRNA fold change
Spleen	Vehicle	35.5 ± 2.6	18.8 ± 1.1	16.9 ± 2.1	–	–
Spleen	THL	22.8 ± 0.7	18.9 ± 0.4	3.9 ± 1.0	13.0	8,192
Liver	Vehicle	33.9 ± 2.8	24.2 ± 1.7	10.0 ± 2.6	–	–
Liver	THL	26.4 ± 0.9	24.3 ± 1.3	2.1 ± 1.0	7.9	238
Brain	Vehicle	37.0 ± 1.4	21.4 ± 0.4	15.9 ± 1.2	–	–
Brain	THL	29.6 ± 0.8	22.1 ± 1.6	7.5 ± 1.8	8.4	338

ΔCq = (NPC1 Cq – GAPDH Cq); ΔΔCq = [ΔCq (vehicle mice)—ΔCq (THL mice)]; NPC1 mRNA fold change between vehicle mice and THL mice computed from base two anti-log of ΔΔCq. Cq values are mean ± SD [eight mice (four males; four females)] in vehicle group; 8 mice [(4 males; 4 females)] in THL group. Reproduced with permission of Jiang et al. (54). GAPDH, glyceraldehyde 3′-phosphate dehydrogenase.